Tracking Axonal Transport of Organelles in Motor Neurons Using a Microfluidic Device

Source: Altman, T., et al. Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System. J. Vis. Exp. (2020). This video demonstrates a method for tracking axonal transport in motor neurons using a microfluidic chamber system. It involves culturing mouse embryonic spinal cord tissues in polymer-coated wells with nutrient medium to induce axonal growth. The movement of fluorescent dye-stained organelles in live imaging, confirms the bidirectional axonal transport.