Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear

Source: Iyer, M. R. et al., Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings. J. Vis. Exp. (2023). This video showcases a method for isolating vestibular neurons from a mouse pup's inner ear. It meticulously outlines the steps involved in dissecting the inner ear, harvesting the vestibular ganglion, and subsequently dissociating the ganglion into single cells. These cells are then cultured on glass-bottom dishes to establish a vestibular neuron culture.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31089
Primary Topic
Hearing, Cochlea, Tinnitus, Genetics
Type
article
Field-Weighted Citation Impact
0.00
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article

Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear

Journal of Visualized Experiments
Hearing, Cochlea, Tinnitus, Genetics
article

Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear

article en

Abstract

Source: Iyer, M. R. et al., Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings. J. Vis. Exp. (2023). This video showcases a method for isolating vestibular neurons from a mouse pup's inner ear. It meticulously outlines the steps involved in dissecting the inner ear, harvesting the vestibular ganglion, and subsequently dissociating the ganglion into single cells. These cells are then cultured on glass-bottom dishes to establish a vestibular neuron culture.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 82%
Hearing, Cochlea, Tinnitus, Genetics
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Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS