A Technique to Optimize Cerebral Organoid Culture Using Lateral Soft Light Illumination

Source: Chen, B. et al., Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination. J. Vis. Exp. (2022). This video showcases the utilization of a lateral soft light illumination technique to improve the naked-eye visualization of embryoid bodies, thereby facilitating their manipulation and transfer during cerebral organoid culture. It elucidates the setup of soft light illumination, methods for media changes and embryoid body transfer, as well as the procedure for inducing neural differentiation in embryoid bodies for cerebral organoid development.