Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays

Source: Hahnewald, S., et al Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays. J. Vis. Exp. (2016) This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.