Differentiation of Neural Stem Cells to Neurons Using a Compartmentalized Microfluidic Device

Source: Paranjape, S. R., et al. Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips. J. Vis. Exp. (2019) The video demonstrates the differentiation of neural stem cells (NSCs) using a microfluidic device comprising a somatic and an axonal compartment separated by a microgroove barrier. NSCs are introduced into the somatic compartment and are allowed to adhere. The NSCs differentiate into neurons inside the somatic compartment and extend axons through the microgroove barrier to the axonal compartment.