Inducing Axonal Varicosities with a Micromechanical Stimulus on Neurons

Source: Servello, D., et al. A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons. J. Vis. Exp. (2018). This video demonstrates the induction of axonal varicosities by applying micromechanical stimulus on neurons. The system comprises a micropipette attached to a syringe containing buffer via tubing. Using a micromanipulator, the micropipette is positioned above the neurons. Fluid pressure is then applied to induce varicosities in axons.