Isolation of Cells from Sensory Ganglia

Source: Kaelberer, M. M., et al. A Method to Target and Isolate Airway-innervating Sensory Neurons in Mice. J. Vis. Exp. (2016). This video demonstrates the isolation of cells from the sensory ganglia of mice. Ganglia are subjected to enzymatic digestion to dissolve the extracellular matrix. Single cells are then collected via filtering through a cell strainer followed by density gradient centrifugation for debris removal. Finally, washing and centrifugation are done to collect the isolated cells for future analysis.