Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse

Source: Wang, Z. et al., Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse. J. Vis. Exp. (2018). This video demonstrates a method for isolating enteric glial cells from the submucosa and lamina propria of the mouse intestine. It outlines the steps involved in extracting submucosa and lamina propria tissue, isolating the enteric glial cells from them, and culturing the cells on a coated well-plate to establish an enteric glial cell culture.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31102
Primary Topic
Gastrointestinal motility and disorders
Type
article
Field-Weighted Citation Impact
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article

Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse

Journal of Visualized Experiments
Gastrointestinal motility and disorders
article

Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse

article en

Abstract

Source: Wang, Z. et al., Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse. J. Vis. Exp. (2018). This video demonstrates a method for isolating enteric glial cells from the submucosa and lamina propria of the mouse intestine. It outlines the steps involved in extracting submucosa and lamina propria tissue, isolating the enteric glial cells from them, and culturing the cells on a coated well-plate to establish an enteric glial cell culture.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 71%
Gastrointestinal motility and disorders
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Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS