Preparation of Organotypic Slice Cultures from a Rat Pup's Hippocampus

Source: Mosa, A. J. et al., Organotypic Slice Cultures for Studies of Postnatal Neurogenesis. J. Vis. Exp. (2015). This video demonstrates a method for preparing organotypic hippocampal slice cultures from the brain of a rat pup. It outlines the steps involved in the dissection of the hippocampus, slicing, and culture conditions required to promote cell viability and growth in the hippocampal slices, ultimately generating organotypic slice cultures.