Establishing a Müller Glial Cell Culture Obtained from Mouse Retinas

Source: Kang, S., et al. Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment. J. Vis. Exp. (2022). This video demonstrates the culturing of Müller glial (MG) cells isolated from mouse retinas. Retinas are dissociated into a single-cell suspension using digestive enzymes. Cells are then grown in a medium with a growth factor that promotes MG cell proliferation and neuronal cell mortality. Dead neuronal cells are removed while passaging, and MG cells are grown for further analysis.