Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia

Source: Portela-Lomba, M., et al. Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration. J. Vis. Exp. (2020) This video demonstrates the procedure to isolate retinal ganglion neurons (RGNs) from a rat's retina and coculture them with olfactory ensheathing glia (OEG) cells. This method allows for studying the neuro-regenerative potential of OEGs after neural injury.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31080
Primary Topic
Olfactory and Sensory Function Studies
Type
article
Field-Weighted Citation Impact
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article

Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia

Journal of Visualized Experiments
Olfactory and Sensory Function Studies
article

Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia

article en

Abstract

Source: Portela-Lomba, M., et al. Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration. J. Vis. Exp. (2020) This video demonstrates the procedure to isolate retinal ganglion neurons (RGNs) from a rat's retina and coculture them with olfactory ensheathing glia (OEG) cells. This method allows for studying the neuro-regenerative potential of OEGs after neural injury.

Journal of Visualized Experiments
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Olfactory and Sensory Function Studies
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Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS