Generating a Blood-Brain Barrier Chip Using iPSC-derived Precursor Cells

Source: Jagadeesan, S., et al., Generation of a Human iPSC-Based Blood-Brain Barrier Chip. J. Vis. Exp. (2020). This video showcases creating a blood-brain barrier chip using iPSC-derived precursor cells. It involves seeding neural progenitor cells and brain microvascular endothelial cells in separate channels with a porous membrane. By flowing differentiation media, the endothelial cells form tight junctions resembling blood vessels, and the neural cells mature into various brain cell types, establishing a functional blood-brain barrier on the chip.