Microelectrode Array-Based Assessment of Neuronal Networks in Mouse Spinal Cord Slices

Source: Iredale, J. A., et al. Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays. J. Vis. Exp. (2022). This video demonstrates a microelectrode array-based assay for studying neuronal network activity in mouse spinal cord sections. First, the electrophysiological activity from the superficial dorsal horn (SDH) of the slice is recorded. Then, a potassium channel inhibitor is introduced to prolong depolarization, resulting in synchronous rhythmic activity across the neuronal network.