Dissociating and Culturing Neurons from Hippocampal Tissue Samples

Source: Servello, D. et al., A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons. J. Vis. Exp. (2018). This video demonstrates the dissociation and culture of hippocampal neurons from hippocampal tissue samples using enzymatic and mechanical dissociations. The treatment with Ara-C inhibits the growth of non-neuronal cells, facilitating the selective growth of hippocampal neurons.