Culturing Fluorescent Excitatory Neurons from a Transgenic Mouse Pup Brain

Source: Turko, P., et al. Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting. J. Vis. Exp. (2019) The video illustrates a technique for culturing fluorescent GABAergic and glutamatergic neurons from the cortex and hippocampal tissues of transgenic mouse pups. The process includes obtaining purified single-cell suspensions of neurons, sorting GABAergic neurons based on their fluorescence protein expression, and seeding them onto poly-L-lysine-coated plates to facilitate attachment and cell proliferation.