Generating Neural Progenitors from Embryonic Stem Cells in Serum-Free Monolayer Cultures

Source: Wongpaiboonwattana, W., et al. Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture. J. Vis. Exp.(2015) The video demonstrates a protocol that uses serum-free media to generate neural progenitors from mouse embryonic stem cells. The desired concentration of embryonic stem cells, suspended in serum-free media, is plated on gelatin-coated coverslips. At an optimal cell density, cells produce autocrine signaling factors, which, along with the neural differentiation-inducing factors in the serum-free media, trigger the differentiation of embryonic stem cells into neural progenitors.