Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting

Source: Mussa, Z., et al. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (2021) This video demonstrates the procedure for isolating and sorting nuclei from fresh-frozen human brain tissue using fluorescence-activated nuclei sorting (FANS), which enables the study of different cell populations for various neurological applications.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31138
Primary Topic
Cell Image Analysis Techniques
Type
article
Field-Weighted Citation Impact
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article

Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting

Journal of Visualized Experiments
Cell Image Analysis Techniques
article

Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting

article en

Abstract

Source: Mussa, Z., et al. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (2021) This video demonstrates the procedure for isolating and sorting nuclei from fresh-frozen human brain tissue using fluorescence-activated nuclei sorting (FANS), which enables the study of different cell populations for various neurological applications.

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Cell Image Analysis Techniques
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Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS