Developing a Triple Cell Culture Model of the Human Blood-Brain Barrier
Source: Fattakhov, N., et al. A triple primary cell culture model of the human blood-brain barrier for studying ischemic stroke in vitro. J. Vis. Exp. (2022) This video demonstrates the co-culturing of human brain pericyte, astrocyte, and endothelial cells. Each cell layer is formed separately and then cultured together, creating a three-layered system that mimics the cellular arrangement of the blood-brain barrier
Publication Details
- Journal
- Journal of Visualized Experiments
- Published
- 2025-07-24
- DOI
- https://doi.org/10.3791/31061
- Primary Topic
- Barrier Structure and Function Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00