Developing a Triple Cell Culture Model of the Human Blood-Brain Barrier

Source: Fattakhov, N., et al. A triple primary cell culture model of the human blood-brain barrier for studying ischemic stroke in vitro. J. Vis. Exp. (2022) This video demonstrates the co-culturing of human brain pericyte, astrocyte, and endothelial cells. Each cell layer is formed separately and then cultured together, creating a three-layered system that mimics the cellular arrangement of the blood-brain barrier

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31061
Primary Topic
Barrier Structure and Function Studies
Type
article
Field-Weighted Citation Impact
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article

Developing a Triple Cell Culture Model of the Human Blood-Brain Barrier

Journal of Visualized Experiments
Barrier Structure and Function Studies
article

Developing a Triple Cell Culture Model of the Human Blood-Brain Barrier

article en

Abstract

Source: Fattakhov, N., et al. A triple primary cell culture model of the human blood-brain barrier for studying ischemic stroke in vitro. J. Vis. Exp. (2022) This video demonstrates the co-culturing of human brain pericyte, astrocyte, and endothelial cells. Each cell layer is formed separately and then cultured together, creating a three-layered system that mimics the cellular arrangement of the blood-brain barrier

Journal of Visualized Experiments
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Barrier Structure and Function Studies
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Developing a Triple Cell Culture Model of the Human Blood-Brain Barrier · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS