Isolation and Culture of Mouse Primary Cerebellar Granule Neurons

Source: Laaper, M., et al. Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons. J. Vis. Exp. (2017) This video demonstrates the technique for isolating and culturing primary mouse cerebellar granule neurons. Cells are treated with an antimetabolite to eliminate proliferating glial cells and produce a pure culture of cerebellar granule neurons.