A Method for the Isolation of Neurovascular Units from Rat Brain

Source: Brzica, H., et al,. A simple and reproducible method to prepare membrane samples from freshly isolated rat brain microvessels. J. Vis. Exp. (2018). This video demonstrates the process of isolating brain microvessels from euthanized rats. It includes the dissection of the brain, cleaning of the tissue, and mechanical digestion, followed by repeated centrifugation to isolate the pure fraction of microvessels.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31104
Primary Topic
S100 Proteins and Annexins
Type
article
Field-Weighted Citation Impact
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article

A Method for the Isolation of Neurovascular Units from Rat Brain

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Journal of Visualized Experiments
S100 Proteins and Annexins
article

A Method for the Isolation of Neurovascular Units from Rat Brain

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article en

Abstract

Source: Brzica, H., et al,. A simple and reproducible method to prepare membrane samples from freshly isolated rat brain microvessels. J. Vis. Exp. (2018). This video demonstrates the process of isolating brain microvessels from euthanized rats. It includes the dissection of the brain, cleaning of the tissue, and mechanical digestion, followed by repeated centrifugation to isolate the pure fraction of microvessels.

Journal of Visualized Experiments
Openalex Percentile: Top 83%
S100 Proteins and Annexins
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