Isolating Murine Retinal Ganglion Cells Using Fluorescence-Activated Cell Sorting

Source: Chintalapudi, S. R., et al. Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry. J. Vis. Exp. (2017) This video demonstrates the procedure for isolating murine primary retinal ganglion cells (RGCs) by using flow cytometry. This method allows for the future study of RGCs, with the goal of better understanding the major decline in visual acuity that results from the loss of functional RGCs in neurodegenerative diseases.