Analyzing Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

Source: Naomi Keliinui, C., et al. Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants. J. Vis. Exp. (2022). This video demonstrates a protocol to identify exogenous factors that initiate the reactivation of Drosophila brain neural stem cells from their quiescent state. Freshly hatched larvae are dissected to isolate their brains, which are then cultured in media with or without a test hormone to evaluate the hormone's potential to induce neural stem cell reactivation and proliferation.