Generating Cortical Pyramidal Neurons from Human Neural Stem Cells

Source: Gouder, L., et al,. Three-dimensional quantification of dendritic spines from pyramidal neurons derived from human induced pluripotent stem cells. J. Vis. Exp. (2015) This video describes a method for preparing glass coverslips coated with polyornithine and laminin to enhance the adhesion and growth of neural stem cells. The technique involves sequential incubations and washes, facilitating the formation of a supportive matrix that promotes neuronal cell growth and differentiation, mimicking structures found in the neurons.