Preparing Free-Floating Slice Cultures from an Adult Human Brain

Source: Fernandes, A., et al. Short-Term Free-Floating Slice Cultures from the Adult Human Brain. J. Vis. Exp. (2019). This video demonstrates a technique for preparing free-floating slice cultures from the adult human brain. Thin slices are generated from a brain section using a vibratome. These slices are then suspended in a culture medium supplemented with growth factors to maintain tissue viability.