Generation of Primary Retinal Ganglion Cell Cultures From Zebrafish Embryos

Source: Terzi, A., et al., ROS Live Cell Imaging During Neuronal Development. J. Vis. Exp. (2021) The video demonstrates a method for culturing primary retinal ganglion cells from zebrafish embryos. Early-stage embryos undergo dissection to isolate the eyes, which are subsequently mechanically dissociated into a suspension of retinal ganglion cells. This cell suspension is cultured on coverslips coated with adhesive proteins to facilitate axon development.