Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos

Source: Whitman, M. C., et al. Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth. J. Vis. Exp. (2019) This video demonstrates the ex vivo culture and imaging of oculomotor slices from transgenic mouse embryos. The embryos are isolated and embedded in agarose. Using a vibratome, slices containing the oculomotor nuclei and eyes are obtained. The slices are maintained in culture and imaged using a fluorescence microscope.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31145
Primary Topic
RNA Interference and Gene Delivery
Type
article
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article

Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos

Journal of Visualized Experiments
RNA Interference and Gene Delivery
article

Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos

article en

Abstract

Source: Whitman, M. C., et al. Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth. J. Vis. Exp. (2019) This video demonstrates the ex vivo culture and imaging of oculomotor slices from transgenic mouse embryos. The embryos are isolated and embedded in agarose. Using a vibratome, slices containing the oculomotor nuclei and eyes are obtained. The slices are maintained in culture and imaged using a fluorescence microscope.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 83%
RNA Interference and Gene Delivery
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Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS