A Technique to Generate Brain Organoids from Human Embryonic Stem Cells

Source: Boisvert, E. M., et al. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. J. Vis. Exp  (2020). This video demonstrates a technique for generating cerebral organoids from human embryonic stem cells (hESCs). The adherent culture of hESC colonies is treated with enzymes to detach and break them into clusters. These clusters form three-dimensional spheres in suspension, which are then induced to differentiate into organoids that mimic the brain cortex with layers of neurons and glial cells.