Isolation and Culture of Microglia from Rat Brains using Serum-Free Media

Source: Collins, H. Y., et al., Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium. J. Vis. Exp. (2018) This video demonstrates a method for isolating and culturing microglia from rat brains. It entails separating microglia from other neuronal and non-neuronal cells using plates coated with a monoclonal antibody specific to microglia.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31021
Primary Topic: Neuroinflammation and Neurodegeneration Mechanisms
Type: article
Field-Weighted Citation Impact: 0.00

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article

Isolation and Culture of Microglia from Rat Brains using Serum-Free Media

Jul 24, 2025

Journal of Visualized Experiments

Neuroinflammation and Neurodegeneration Mechanisms

article

Isolation and Culture of Microglia from Rat Brains using Serum-Free Media

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Zero hunger

Openalex Percentile: Top 76%

Neuroinflammation and Neurodegeneration Mechanisms

DOI Source OpenAlex

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Isolation and Culture of Microglia from Rat Brains using Serum-Free Media · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS