Human Pluripotent Stem Cell Differentiation in Neurons Using Engineered Lentiviruses

Source: Assetta, B., et al., Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions. J. Vis. Exp. (2020). This video demonstrates the process of inducing human pluripotent stem cells to differentiate into neurons by transfecting them with engineered lentiviruses carrying a neurogenic transcription factor gene under the control of  tetracycline-responsive element, followed by treatment with a tetracycline derivative and maturation medium.