A Technique for the Prolonged Incubation of Neuronal Tissue of the Retina

Source: Cameron, M. A., et al. Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging. J. Vis. Exp. (2017). This video demonstrates a method for prolonged incubation of neuronal tissue of the retina. Eyecups containing retina are obtained from a mouse eye; the inner limiting membrane (ILM) is enzymatically digested to expose the neuronal cells of the retina, and either the treated eyecup or the isolated retina is transferred to a specialized incubator for prolonged incubation at a low temperature.