A Technique for Generating Neural Progenitor Cells from Mouse Embryonic Stem Cells
Source: Mao, X., et al. Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro. J. Vis. Exp. (2020). This video demonstrates a method for the in vitro generation of neural progenitor cells (NPCs) from mouse embryonic stem cells (mESCs). The mESCs are cultured in suspension to form embryoid bodies (EBs), which are then guided to differentiate into NPCs via retinoic acid treatment in an adherent culture.
Publication Details
- Journal
- Journal of Visualized Experiments
- Published
- 2025-07-24
- DOI
- https://doi.org/10.3791/31054
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00