Generating an Organoid Culture from a Brain Tumor Cell Suspension

Source: Sundar, S. J. et al.  Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture. J. Vis. Exp. (2022). This video describes the preparation and cultivation of brain tumor organoids from single-cell suspension. Using a laminin-rich extracellular matrix as a scaffold and culture media for nutrients, cells are facilitated to multiply and self-organize within the organoid. Consequently, ex vivo organoids are generated, mimicking cellular diversity and the microenvironment of brain tumors.