The Regulation of Paxillin on the Proliferation and Migration of PASMCs Induced by the Serum of Rats with HPS

目的：评价桩蛋白(Paxillin)在肝肺综合征(HPS)大鼠血清诱导肺动脉平滑肌细胞(PASMCs)增殖和迁移中的作用。方法：选取20只雄性Sprague-Dawley大鼠，运用慢性胆总管结扎法构建HPS大鼠模型，并采集腹主动脉血样制备血清。将培养的大鼠PASMCs分别接种于6孔板、24孔板和96孔板，通过随机数字表法均分为2组：对照组(C组)和HPS组。C组添加正常大鼠血清，HPS组加入HPS大鼠血清，使两组血清终浓度均为5%。于细胞孵育24 h (T1)、48 h (T2)和72 h (T3)，采用RT-PCR法和Western blot法测定PASMCs内paxillin mRNA及其蛋白的表达水平；运用3H-TdR法检测PASMCs的增殖水平，借助Transwell小室(T1)及划痕实验(T1~T3)测定PASMCs的迁移水平。结果：与C组相比，HPS组PASMCs的增殖和迁移能力显著增强(P Objective: To explore the mechanism of action of paxillin in the proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) induced by the serum of rats with hepatopulmonary syndrome (HPS). Methods: Twenty male Sprague-Dawley rats were selected. The HPS rat model was established by chronic common bile duct ligation, and abdominal aortic blood was collected to prepare serum. The cultured rat PASMCs were seeded into 6-well, 24-well, and 96-well plates respectively, and randomly divided into a control group (Group C) and an HPS group using the random number table method. Group C was supplemented with normal rat serum, while the HPS group was added with HPS rat serum, with the final serum concentration adjusted to 5% in both groups. At the time points of 24 h (T1), 48 h (T2), and 72 h (T3) of cell incubation, RT-PCR and Western blot were used to determine the expression levels of paxillin mRNA and protein in PASMCs. The 3H-TdR method was employed to detect the proliferation level of PASMCs, and the Transwell chamber (T1) and scratch assay (T1~T3) were used to measure the migration level of PASMCs. Results: Compared with Group C, the proliferation and migration abilities of PASMCs in the HPS group were significantly enhanced (P < 0.05). In the HPS group, the expression of paxillin mRNA and protein showed a gradually increasing trend with the extension of the stimulation time (P < 0.05). Conclusion: Stimulation by the serum of HPS rats can gradually upregulate the expression of paxillin in PASMCs, which may play a crucial regulatory role in the abnormal proliferation and migration of PASMCs associated with HPS.