PHYTOCHEMICAL CONSTITUENTS WITH ANTIMICROBIAL ACTIVITY FROM ETHANOLIC EXTRACT OF LAWSONIA INERMIS LEAVES AND STEM EXTRACTS

Medicinal plants have been used for decades against microbial diseases.Phytochemicals are used in the pharmacological industry as a natural substitute due to their potential biological activities, including antimicrobial activity.The present study extracted Henna leaves and stems using ethanol and n-hexane.These extracts were assessed for antimicrobial activity using the disc diffusion method and compared with the antibiotic gentamycin as a positive control.Henna extracts were investigated against six clinical bacterial strains, including two Gram-positive strains, viz.Bacillus cereus and Staphylococcus aureus and four Gram-negative viz.E. coli, S. typhi, P. aeruginosa, A. baumannii.The antifungal activity of henna was evaluated against four fungal strains viz.Aspergillus niger, Aspergillus flavus, Aspergillus paracitus, and Rhizopus stolonifer.The results of antibacterial activity revealed that the ethanol extract of henna leaves and stems reported maximum activity against S. aureus, while minimum activity was shown against A. baumanni.The ethanol extract of henna leaves and stems showed remarkable antifungal activity against A. paracitus and R. stolonifer, respectively.The High-performance liquid chromatography analysis revealed that the ethanol extract of henna was composed of ten phytochemicals.The results of this study showed that henna is potent with the antimicrobial component.