Association between Plasma Testosterone Concentration and Semen Quality Parameters in Kokok Balenggek Roosters

This study investigated the association between plasma testosterone concentration and semen quality parameters in Kokok Balenggek roosters (KBR), a native Indonesian breed renowned for its distinctive crowing.Fifteen roosters aged 1-2 years were used, and blood and semen samples were collected four times at 10-day intervals, yielding 60 total samples.Plasma testosterone concentrations were measured using a competitive.At the same time, semen quality was evaluated through macroscopic (volume, pH, color, odor, enzyme-linked immunosorbent assay (ELISA).In contrast, semen quality was evaluated through both macroscopic (volume, pH, color, odor, and consistency) and microscopic (mass movement, motility, viability, abnormality, and sperm concentration) assessments.The mean plasma testosterone concentration was 18.907.27ng/mL.Semen characteristics showed an average volume of 292.75143.76L,pH of 6.960.10,motility of 82.777.04%,viability of 88.941.62%,abnormality of 9.022.86%,and sperm concentration of 2630696.4210cells/mL.Plasma testosterone exhibited weak and non-significant correlations with semen volume (r=-0.369),pH (r=0.400),mass movement (r=0.081),motility (r=0.075),viability (r=0.025),sperm concentration (r=0.134), and abnormality (r=-0.267).These findings suggest that plasma testosterone concentration alone does not strongly influence semen quality in Kokok Balenggek roosters, highlighting the need to consider additional physiological and environmental factors for optimizing reproductive performance.