Seminal Plasma Protein Profiles Based on Molecular Weight and Their Relationship with Sperm Quality in Kokok Balenggek Roosters

This study aimed to characterize the seminal plasma protein profiles of Kokok Balenggek roosters and investigate their relationship with semen quality parameters.Semen samples were collected from 15 roosters and analyzed for motility, viability, abnormality, and intact plasma membrane (IPM).Seminal plasma proteins were separated by SDS-PAGE, and molecular weights were estimated.The results showed that the 51kDa protein band exhibited strong positive correlations with sperm motility, viability, and IPM, and a significant negative correlation with sperm abnormality.These findings suggest that the 51kDa protein, possibly clusterin, plays a crucial role in sperm function and may serve as a potential fertility biomarker in indigenous poultry.The study highlights the importance of seminal plasma protein profiling in improving reproductive performance and selection strategies for local poultry breeds.Further studies are recommended to identify the specific proteins involved and their biological functions.