Development of Single component (Piperine) Fingerprint by UV Spectroscopy and HPTLC for Sitopaladi Churna: An Official Ayurvedic formulation

Sitopaladi churna is an Official Ayurvedic formulation (AFI, 2000a) which is recommended in treatment of varied ailments. Mainly it is used in treatment of Neuralgia, asthma, digestive impairment, etc. Due to various side effects of available allopathic medicines, now a days, demand and usage of Traditional medicines have increases tremendously. For global acceptance of Indian Traditional system we have to develop various standardization methods for our Ayurvedic formulations with available sophisticated instruments. Till date no such method was established for standardization of Sitopaladi churna. We have tried to develop a robust method and standardized Sitopaladi churna mainly fingerprinting as it is very economic in terms of time and machinery usage. Present study was undertaken for fingerprinting of selected formulations and raw materials using UV and HPTLC techniques. HPTLC study was carried out on Sitopaladi churna and raw material extracts using Camag HPTLC system equipped with Linomat V applicator, camag TLC scanner 3 and CATS 4 software for interpretation of data. One in house preparation (SiCL) and two marketed preparation (SiCM-I and SiCM-II) of Sitopaladi Churna were subjected for development of reproducible, rapid, valid, robust fingerprinting method.