Comparative Immunological Studies for Evaluation Enterotoxemia Vaccine in Rabbit

Enterotoxemia caused by Clostridium perfringens is an important disease in rabbits.Clostridium perfringens type A induces severe diarrhea, bloat, and high mortalities, especially among weaned rabbits.This study investigated the potency of inactivated vaccine formulations that protect rabbits against Clostridium perfringens type A by preparing two monovalent vaccines with different adjuvants (Montanide gel 01 TM and aluminum hydroxide gel).Three groups of rabbits consisted of a control group that received 2mL phosphate buffer saline subcutaneously and two groups injected with 2mL of the prepared monovalent vaccines subcutaneously in 2 doses at three weeks intervals with two different adjuvants (Montanide gel and aluminum hydroxide gel).Antibody titers of the vaccinated rabbits were determined up to 11 months using ELISA and serum neutralization tests.The aluminum hydroxide gel vaccine was protected till 6 months with a protection rate of 80%, and the Montanide gel 01 TM vaccine was protected till 10 months with a protection rate of 90%.It is concluded that the Montanide gel adjuvant vaccine has a longer-lasting immunity than the aluminum hydroxide gel vaccine.