The Comparison of IL-12 Gene Expression Analysis in the use of Cold Atmospheric Nitric Oxide Alone and Combination with NPH Insulin on the Full-thickness Excisional Wound Healing in a Diabetic Rat Model

This study was designed to compare the effects of different treatments on the expression of the IL-12 gene and its role in the healing of diabetic wounds.An experimental approach was employed to evaluate a novel treatment combining atmospheric nitric oxide (NO) and NPH insulin cream, which has been shown to impact IL-12 gene expression and enhance diabetic wound healing.A total of 24 diabetic rat samples were divided into four groups based on the treatment received: a) DC (Diabetic Control), b) DI (Diabetic + Insulin), c) DNO (Diabetic + Nitric Oxide), d) DINO (Diabetic + Insulin + Nitric Oxide) Gene expression analysis of the IL -12 gene was performed using RT-PCR.Comparisons were made among the four groups (DC, DI, DNO, and DINO) to understand better the impact of these treatments on IL-12 mRNA expression.The result was a statistically significant decrease in IL-12 expression in the DINO group compared to the DC group (P=0.0437).Since higher expression of the IL-12 gene is associated with inflammation and chronic wounds, its reduction indicates improved healing.Notably, nitric oxide (NO), which has antimicrobial properties, appeared to suppress IL-12 expression in the DNO group, suggesting that NO contributes to reduced inflammation and promotes faster wound healing.