Antimicrobial Content and Sensitivity Test of Citrus Peel Eco-enzyme as Natural Teat Dipping Solution in Dairy Cattle

Post-milking teat dipping of dairy cattle into antiseptic solutions (Teat dipping) to protect the teats has been widely recognized in various dairy farming countries, which can minimize contamination of the teats from pathogenic bacteria.However, dairy farmers still rely on synthetic antiseptics, where their use can leave suitable residues indirectly in the milk dairy cows produce.The purpose of this study was to obtain the content and dose of citrus fruit peel (Citrus reticulata) eco-enzyme by analyzing phenol, tannin, ethanol, acetic acid and its sensitivity effect on Streptococcus sp.bacteria so that it can be used as a natural teat dipping solution in dairy cows.The method used in this research is an experimental study with a completely randomized design which is seen from the level/dose of Eco-enzyme based on citrus peel, as follows: D1.Synthetic Antiseptic (control), D2.Eco-enzyme solution (30%), D3.Eco-enzyme solution (60%), D4.Eco-enzyme solution (100%).The results of the orange peel eco-enzyme solution research show a significant effect on phenol content (11.3-75.5mgGAE/gr),tannins (0.05-0.89%1000 ppm), ethanol (1.99-2.92%),acetic acid (0.21-0.57%) and inhibition against Streptococcus sp.bacteria (2.87-6.75mm),and it can be concluded that citrus fruit peel (C.reticulata) eco-enzyme solution treatment D4 can be used as a natural antiseptic candidate for teat dipping solution in dairy cows.