Studies of Release Activity in DRGs of Rats with Spared Nerve Injury (SNI) Using YO-PRO-1

We report a new method for examining changes in membrane permeability in dorsal root ganglion (DRG) cells under peripheral nerve injury conditions. By intravenously injecting YO-PRO-1, a monomeric cyanine nucleic acid stain, into rats with spared nerve injury (SNI), we observed the uptake of YO-PRO-1 in injured neurons and surrounding non-neuronal cells. This approach provides a tool for detecting in vivo DRG cell release activity in intact animals.