Evaluation of the neurotropic activity of the phytocomposition «Memoris»

Background . Matters of developing new medicinal drugs with nootropic action require systematization of our knowledge as well as conducting of experimental and clinical research. The aim . Evaluation of the neurotropic activity of the phytocomposition “Memoris”. Materials and methods . Research is performed on male Wistar rats. Results were evaluated after the end of 14-day-old intake of studied phytocomposition and reference drug Cortexin. Neurotropic activity was measured by tests in open field, elevated plusmaze and Morris water maze. Serotonin, noradrenaline and dopamine content were defined by HPLC-MS/MS. Results . Under the influence of “Memoris” composition the significant enhancement of locomotor activity in central zone of the open field apparatus had been achieved. This enhancement manifested in statistically significant increase in number of crossed squares, time of movement and time of immobility within central part. Administration of the phytocomposition increased the time rats spent in open arms and in the center of the plus-maze, increased number of head-dips and decreased number of entrances into enclosed arms. Training in the Morris water maze with hidden platform demonstrated statistically significant decrease of latent period of platform search in rats which received phytocomposition and Cortexin by the second day of testing (in control group rats – by the third day). At the day 2 of testing, the time of platform search was statistically significantly different in the groups of rats which received phytocomposition and Cortexin as compared to the control group. Under the influence of “Memoris” phytocomposition increase of serotonin (by 92 %, р = 0,001) and dopamine (by 57 %, р < 0,001) content in hypothalamus, as well as decrease of noradrenaline (by 17 %, р = 0,002) content in hypothalamus were observed. Conclusion . Phytocomposition “Memoris” has anxiolytic effect comparable to that of Cortexin, increases capabilities for associative learning in rats, affects the concentration of important neurotransmitters – serotonin, dopamine and noradrenaline.