In vitro evaluation of antimicrobial, anti-inflammatory, and cytotoxic properties against MCF 7 cell line of essential oil extracted from Nigella sativa seed

AbstractNigella sativa, often termed black cumin, is claimed to have therapeutic benefits namely bactericidal, anti-inflammatory, and antioxidant properties. Hydro distillation was employed to obtain essential oil that emanates from Nigella sativa seeds. The biologically active elements were identified by employing Mass spectroscopy-gas chromatography. The essential oil’s anti-inflammatory performance were assessed by protein denaturation and trypsin inhibition assays, whereas the DPPH assay was used to assess its antioxidant capacity. Furthermore, MTT assay performed to identify the cytotoxic effects on MCF-7 cell lines. The results demonstrated the presence of key bioactive compounds with promising anti-inflammatory and cytotoxic properties. These findings suggest that N. sativa essential oil holds potential as a therapeutic agent with applications in anti-inflammatory and anticancer treatments.