Choice of approaches to the standardization of the original plant collection “Opornofit”

Actuality.Diseases of musculoskeletal system occupy 3rd place in the world in terms of prevalence.Plant remedies are used in the therapy of such diseases.The aim is to analyse the range of plant collections for the treatment and prevention of diseases of the musculoskeletal system with the isolation of plant raw materials which are most often included in their composition to establish the most promising groups of substances that cause pharmacological action, to confirm/cancel the prospects of their using in the development of methods for quality control of the plant collection "Opornofit species".Material and methods.Content analysis of available sources of information.The component composition of organic acids was determined by gas chromatography-mass spectroscopy and compounds of catechin -by high-performance liquid chromatography.Research results.It was established that the composition of plant remedies and plant collections recommended for pathologies of the musculoskeletal system includes plant raw materials from 89 plant species belonging to 32 families.The most common components of the preparations are herb of Polygonum aviculare L., Hypericum perforatum L. and Thymus serpyllum L.; leaves of Urtica dioica L. and Rubus fruticosus L.; flowers of Crataegus sanquinea Pall.and Matricaria recutita L.; fruits of Rosa majalis Herrm.and Crataegus sanquinea Pall.; roots of Comarum palustre L. and Arctium lappa L. Analysis of the chemical composition of these types of plant raw materials showed that the main factors of pharmacological activity are phenolic compounds and organic acids.Therefore, the study of the qualitative composition of organic acids and catechins in the developed plant collection "Oprnofit" as potentially important groups of biologically active substances for its standardization is relevant.The quantitative content of 8 organic acids and 4 catechins in plant collection was identified and determined.,The highest content was for the citric acid from the identified organic acids which indicates the expediency of recalculating the quantitative determination of the total organic acids on it.Of the identified catechins, the highest content was fort epicatechin and epicatechin gallate, therefore these compounds can be used as marker substances for the identification of polyphenols.Conclusion.As a result of the analysis of the component composition of plant remedies and plant collection which are recommended for diseases of the musculoskeletal system, the types of plant raw materials that are most often included in their composition were established.The chemical composition of these types of plant raw materials was analysed and the most promising groups of substances that determine the pharmacological action of the preparations were established.For the first time, the quantitative content of 8 organic acids and 4 catechins was identified and determined in the original plant collection "Oprnofit".Given the dominance of citric acid content (1 215,74 mg/g), it is rational to quantitatively determine the total organic acids in terms of citric acid.The highest content of epicatechin from the identified catechins (3 301,38 mg/g) makes it possible to use this substance as a marker for identification (section "Identification C" of the quality control methods).The results obtained will be used in the development of quality control methods for the plant collection "Opornofit species".