Biochemical characteristics of pro- and antioxidant potential of plant extracts in dairy environment

Actuality.Due to the high content of antioxidants, vitamins, minerals in plant extracts, their use for inhibiting oxidative processes is gaining popularity.The antioxidant efficacy of aqueous extracts from basil seeds, grape leaves, robinia flowers and wheat grains can be investigated using cow and goat milk as a research medium.The purpose of the study.To determine the content of secondary metabolites in plant raw materials and to evaluate the pro-and antioxidant potential of aqueous plant extracts in dairy environment.Material and methods.Native cow and goat milk.Water extracts from grape leaves (Vitis vinifera L.), robinia flowers (Robinia pseudoacacia L.), basil seeds (Ocimum basilicum L.) and wheat grains (Triticum sp.).The aqueous extracts were prepared in a 3 : 10 ratio, followed by extraction at 90 temperature for 40 minutes.Analysis of secondary metabolites was carried out by reverse phase high performance liquid chromatography.To prepare extracts for HPLC analysis 0,3 g of finely ground materials were mixed with 20 mL of methanol (Merck, Germany) and the obtained mixtures were treated with ultrasound at 50 for 120 min.Syringe filters with PTFE membranes (0,22 m pore size) were used for filtration of obtained extracts.The content of oxidation products of lipids and proteins, vitamin C, SH groups, reduced glutathione, catalase activity and ascorbate peroxidase were determined spectrophotometrically.Plant extracts were added in a volume of 3% of the milk volume..