Construction of an Ectopic GFP Gene Transient Expression Vector and Study on Protein Expression

This study developed a rapid tool for plant gene function research by constructing a transient ectopic expression vector for GFP—an essential reporter gene—and validating its expression in tobacco leaves. Using gene cloning techniques, GFP was inserted into a plant expression vector, transformed into tobacco via Agrobacterium-mediated delivery, and verified through PCR, gel electrophoresis, and enzyme digestion. The system achieved high-efficiency transient GFP expression in tobacco cells, confirmed by distinct green fluorescence under microscopy. Results demonstrate that this method provides rapid, effective technical support for plant gene expression studies, with significant application value; future optimizations may further enhance its stability and expression efficiency for broader molecular biology applications.