GENETIC DIVERSITY OF CURRALEIRO PÉ-DURO USING A NON-INVASIVE DNA EXTRACTION

Curraleiro Pé-Duro is a native bovine found in Central-West and Northeast regions of Brazil. They originated from Bos taurus ibericus acquiring robust phenotypes that allow them to adapt and survive in semiarid region. For the past decades, they have been interbreeding with exotic breeds attempting to increase their economic production. Unfortunately, this strategy produced genomic contamination leading Curraleiro Pé-Duro to risk of extinction. In this study, we aim to evaluate three non-evasive DNA extraction protocols to access genetic diversity of Curraleiro Pé-Duro. First, we tested well-known protocols to extract DNA from hair follicle. Second, we validated the performance using genomic and mitochondrial primers. Then, we confirm that these genome was accessed by ISSR (Inter Simple Sequence Repeat). We found had the best results was obtain by adding Proteinase K to the Chelex reagent, protocol III. In addition, we found that among twenty nine ISSR primers, five of them, successfully generated consistent and reliable polymorphism to access the genetic diversity in this native bovine.