OsBZR4 regulates temperature-dependent embryogenesis in rice
Embryoless rice is valuable for studying early seed development and has great breeding potential, however, related research remains limited. Here, we show that mutations in OsBZR4, encoding brassinazole-resistance 4, lead to 60-100% embryoless seeds across different cultivars. OsBZR4 is specifically expressed at the scutellum-endosperm interface and regulates auxin levels and distribution during early embryo development. OsBZR4 represses the expression of YUC4 and PIN5b. Exogenous auxin and overexpression of YUC4 enhance the embryoless ratio, whereas the auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) decrease it. Higher temperatures increase the embryoless ratio in bzr4 mutant lines. The expression of OsPIL13 is induced by high temperature, and its overexpression enhances the embryoless ratio in both wild type and bzr4 plants. Introducing bzr4 mutant allele into some elite cultivars can improve milled rice yield and storability. Collectively, manipulating OsBZR4 can lead to the development of thermosensitive embryoless rice varieties with increased energy reserves and improved storability, potentially enabling novel rice production technologies.
Authors
- Xiaoming Zheng (ORCID: https://orcid.org/0000-0003-0941-9089)
- Mingliang He (ORCID: https://orcid.org/0000-0001-6105-5433)
- Yingxiang Liu (ORCID: https://orcid.org/0000-0002-4006-0695)
- Zhipeng Hong (ORCID: https://orcid.org/0009-0006-4700-8829)
- Min Xu (ORCID: https://orcid.org/0000-0002-7206-0059)
- Xiaojie Tian (ORCID: https://orcid.org/0000-0001-9040-5156)
- Qingyun Bu (ORCID: https://orcid.org/0000-0002-5386-3577)
- Zhenyu Wang (ORCID: https://orcid.org/0009-0007-7927-6753)
- Xiufeng Li (ORCID: https://orcid.org/0000-0001-6598-2277)
- Qian Qian (ORCID: https://orcid.org/0000-0002-0349-4937)
- Xin Jin (ORCID: https://orcid.org/0000-0002-9426-1775)
- Jiaqi Tang (ORCID: https://orcid.org/0000-0002-3144-0194)
Institutions
- Northeast Institute of Geography and Agroecology (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Institute of Crop Sciences (CN)
- China National Rice Research Institute (CN)
- University of Chinese Academy of Sciences (CN)
- Yuxian People's Hospital (CN)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-07-26
- DOI
- https://doi.org/10.1038/s41467-025-62262-3
- Citations
- 3
- Primary Topic
- Plant Molecular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 5.25
Funders
- National Natural Science Foundation of China
- Guizhou University
- Anhui Agricultural University
- Huazhong Agricultural University
- National Key Research and Development Program of China