Establishment of a low-temperature immersion method for extracting high-activity and high-purity mitochondria from Syntrichia caninervis Mitt.

The optimized mitochondrial isolation protocol presented here is both time efficient and highly reproducible, yielding mitochondria of exceptional purity suitable for mechanistic studies in desiccation-tolerant mosses. The isolated mitochondria exhibit robust functional activity and structural integrity, providing a reliable platform for investigating stress resistance mechanisms in S. caninervis and other extremophytic species. By establishing a standardized workflow for mitochondrial isolation in desiccation-tolerant plants, this method addresses a critical technical gap and paves the way for advanced investigations into mitochondrial biology under extreme environmental conditions.