Wound Healing Effect of Methanol Extract of Knop Grass (Hyptis capitata Jacq.) on Male Mice

Wound healing is a complex physiological process that can be delayed by infection and inflammation. Hyptis capitata Jacq., known locally as Knop grass, contains phytochemicals such as flavonoids, tannins, alkaloids, saponins, and steroids, which possess antibacterial and anti-inflammatory properties. This study aimed to evaluate the effect of methanol extract of H. capitata leaves on the healing of incisional wounds in male mice (Mus musculus). Twenty mice were divided into four groups: a positive control (10% povidone iodine) and three treatment groups receiving 10%, 20%, and 30% methanol extract topically. Wounds were observed daily, and healing duration was recorded. Phytochemical screening confirmed the presence of active secondary metabolites in the extract. The results showed a concentration-dependent effect on healing time, with the 30% extract group healing significantly faster (9.60 ± 0.55 days) compared to the control (14.00 ± 0.00 days). One-way ANOVA and LSD post hoc analysis confirmed significant differences between all groups (p 0.001). In conclusion, the methanol extract of H. capitata leaves effectively accelerated wound healing in mice, with the 30% concentration being the most effective. Further studies involving histological evaluation and formulation development are recommended to support its potential as a standardized topical wound-healing agent.